We narrowed to 12,952 results for: sequence

1. pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

   Plasmid

   #133026

   Purpose

   Expresses EGFP-tagged tail anchor sequence of Bik

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Bcl-2-interacting killer (BIK Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-110

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-cryR;dll4CE2-P1Egfp

   Plasmid

   #90147

   Purpose

   contains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   dll4E2P1Egfp (dll4 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL718

   Plasmid

   #140388

   Purpose

   Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mobO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Q842gp120-8his

   Plasmid

   #100932

   Purpose

   Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (Q842.d12) Env
   

   Use

   Tags

   CD5 leader sequence and Purification tag after K5…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK2_024

   Plasmid

   #123716

   Purpose

   Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pPGK1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Crippled Kozac sequence

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5xtetO (tet3) Gene Desert

   Plasmid

   #131339

   Purpose

   gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Desert-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. P12p-bio

   Plasmid

   #47726

   Purpose

   Expresses enzymatically monobiotinylated full-length P12p ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised P12p
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. HD201: pMVP (L3-L2) IRES-eGFP + polyA

   Plasmid

   #121747

   Purpose

   pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   IRES2-eGFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. RON3-bio

    Plasmid

    #47799

    Purpose

    Expresses enzymatically monobiotinylated full-length RON3 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RON3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP6-bio

    Plasmid

    #47732

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP6
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RAP2-bio

    Plasmid

    #47795

    Purpose

    Expresses enzymatically monobiotinylated full-length RAP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAP2
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MTK3a_006

    Plasmid

    #123759

    Purpose

    Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ETS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS315-0nt-telo-I-SceI

    Plasmid

    #67624

    Purpose

    telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    3' gal1 unique sequence
    ura4 (ura4 Fission Yeast)
    telomere cassette and I-SceI cut site
    hph
    

    Use

    Yeast cen/ars plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid

    #24417

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Rs1
    

    Use

    Entry vector

    Tags

    FLAG and mCherry

    Expression

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPATR-bio

    Plasmid

    #47782

    Purpose

    Expresses enzymatically monobiotinylated full-length SPATR with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised SPATR
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTH397-SUP45-I222S

    Plasmid

    #29388

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    I222S mutant gene (corresponding to the sup45-2 a…

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128342

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna7 (Chrna7 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28(a)-hFX-HA

    Plasmid

    #14991

    Depositor

    Michael Ristow

    Article

    Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

    Insert

    human frataxin cDNA (FXN Human)
    

    Use

    Tags

    AU1, HA, and HIS

    Expression

    Bacterial

    Mutation

    contains cDNA coding sequence for amino acids 88-…

    Promoter

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

21. ETRAMP10.2-bio

    Plasmid

    #47789

    Purpose

    Expresses enzymatically monobiotinylated full-length ETRAMP10.2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised ETRAMP10.2
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RAP3-bio

    Plasmid

    #47796

    Purpose

    Expresses enzymatically monobiotinylated full-length RAP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAP3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47451

    Purpose

    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NG_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47448

    Purpose

    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NI_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47449

    Purpose

    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NN_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47447

    Purpose

    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NG_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HOXD12 (murine) HIS-tag pET

    Plasmid

    #8553

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pCEP4-HA-Flag-natT1R2 ECD

    Plasmid

    #113954

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-568

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTH399-SUP45-S30P

    Plasmid

    #29372

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S30P mutant gene (corresponding to the sl23ts all…

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pSLQ1371-sgYAP-2

    Plasmid

    #121423

    Purpose

    sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgYAP-2
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge