We narrowed to 25,004 results for: CHI
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Plasmid#99505Purpose3rd generation lentiviral expression of wild-type Becn1DepositorAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGMutationChanged F79 to GAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-PD-L1 4NQ
Plasmid#121487PurposeExpression of human PD-L1 4NQ with PD-L1 shRNADepositorInsertsPD-L1 4NQ
hPD-L1 shRNA
UseLentiviralTagsFlagExpressionMammalianMutationN35Q, N192Q, N200Q and N219QPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 CARD-VC155
Plasmid#49259PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertCaspase-2 CARD (CASP2 Human)
TagsHA and split Venus CExpressionMammalianMutationdeleted aa 123-435PromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hPhyB621-mCherry-HRasCT
Plasmid#100282PurposeExpresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (1-621 aa) (PHYB Mustard Weed)
TagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-621 aa)PromoterCAG promoterAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC591 - pCMV Mem-iRFP
Plasmid#59134PurposeA plasmid that express membrane-targeted iRFP under the CMV promoter.DepositorInsertMembrane-localized Infrared Fluorescent Protein
TagsPalmitylation signalExpressionMammalianPromoterCMV-IEAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1Balpha tail 387-1150
Plasmid#134619PurposeMammalian expression of mouse KIF1Balpha 387-1150DepositorInsertKIF1Balpha (Kif1b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 386 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 CARD-VN173
Plasmid#49260PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 CARD (CASP2 Human)
TagsFlag and split Venus NExpressionMammalianMutationdelete aa 123-435PromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG IL13-CAR
Plasmid#157742Purposeknock IL13-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertChimeric antigen receptor targeting IL13
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE-BI GFP10-RHOB/RBD-GFP11
Plasmid#182231PurposeInducible coexpression of GFP10-RHOB and RBD-GFP11 domainDepositorInsertsUseLentiviralTagsGFP10 (split-GFP tripartite) and GFP11 (M4)PromoterTRE bidirectionalAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro
Plasmid#192682PurposeLentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shD
Plasmid#21964DepositorAvailable SinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn2
Plasmid#59358PurposeLentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorUseLentiviralExpressionMammalianPromoterCAG and mouse U6Available SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS423-mitoT
Plasmid#174525PurposeExpresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranesDepositorInsertYeast mitochondrial intermembrane tether
UseSynthetic BiologyTagseGFPExpressionYeastPromoterMET25Available SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NOPLight-ctr
Plasmid#195579PurposeExpresses the control sensor NOPLight-ctr in mammalian cellsDepositorInsertNOPLight-ctr
ExpressionMammalianMutationD110A, D130APromoterCMVAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
TagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pEYFP_C1
Plasmid#85766Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
TagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514)PromoterCMVAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
TagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6
Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only