We narrowed to 11,283 results for: VARS

1. LV_EF1a_CDK1-P2A-Hygro_Barcode

   Plasmid

   #170221

   Purpose

   Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTFP1-Farnesyl-5

   Plasmid

   #55481

   Purpose

   Localization: Plasma Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pPGK-T7/2-CD44v6-10 (-cyt)

   Plasmid

   #137821

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast SHMT2 CD

   Plasmid

   #106302

   Purpose

   Expresses SHMT2 K280A catalytic site mutant

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K280A catalytic site mutation, Silent mutations d…

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GFAP.SF-iGluSnFR.S72A

   Plasmid

   #106194

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   GltI: S72A

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK2-E2F1 3'UTR

   Plasmid

   #29468

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F1 3'UTR (E2F1 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. psiCHECK2-p27Kip1 3'UTR

   Plasmid

   #29476

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   p27 Kip1 3'UTR (CDKN1B Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

   Plasmid

   #190898

   Purpose

   Entry vector for multiple sgRNA cloning

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6 and 7sk

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Expression

   Worm

   Promoter

   nlp-1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SopB-EGFP

    Plasmid

    #183655

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Δ18 B.1.429

    Plasmid

    #169461

    Purpose

    Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Δ18 B.1.1.298

    Plasmid

    #169460

    Purpose

    Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_ARHGAP32-1/1

    Plasmid

    #91312

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP32-1/1 (ARHGAP32 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

    Plasmid

    #69052

    Purpose

    lentiviral expression of IKZF2 variant 2 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    UBC

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_ABL1-1/1

    Plasmid

    #91521

    Purpose

    Protein expression and purification of human SH3 domain construct ABL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ABL1-1/1 (ABL1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

    Plasmid

    #184335

    Purpose

    Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha-GPI (Nrxn3 Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-SFFV-mtagBFP-2A RIGI K270A

    Plasmid

    #167290

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    K270A

    Promoter

    SFFV

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-T0-Flag-HROB

    Plasmid

    #135298

    Purpose

    Expresses Flag-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHH0103_ASAP2-1/1

    Plasmid

    #91504

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP2-1/1 (ASAP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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