We narrowed to 8,355 results for: BLI
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Plasmid#67013PurposeExpresses malE-ELP[V-60] in E. coliDepositorInsertmalE-ELP[V-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA
Plasmid#51094PurposeCre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophoreDepositorInsertoChIEF(E163A/T199C)
UseAAVTagsP2A-dTomatoExpressionMammalianMutationE163A/T199CPromoterEF1aAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
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4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-ApaLID211A-HA
Plasmid#211459PurposeExpress mitochondrial localized ApaLI with D211A mutationDepositorInsertApaLI-D211A
UseTagsHAExpressionMammalianMutationD211APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-ARNTL-shRNA3
Plasmid#166915PurposeLentivirus for inducible knockdown of human ARNTL(BMAL1)DepositorInsertARNTL shRNA (BMAL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV IGF1-FLAG
Plasmid#175170PurposeLentiviral expression of human Igf1-FLAGDepositorInsertIgf1 (IGF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
UseTagsExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS Omomyc-Mer
Plasmid#113170PurposeExpression of Omomer (tamoxifen inducible Omomyc) in mammalian cellsDepositorInsertOmomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
UseTagsExpressionMammalianMutationE57T, E64I, R70Q, R71NPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Strep2
Plasmid#119753PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.DepositorTypeEmpty backboneUseTagsGST and Strep-tag II fusion tagExpressionBacterialMutationPromotertac promoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-14-3-3 beta GST
Plasmid#13276DepositorInsert14-3-3 beta (YWHAB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pLEX307-TMPRSS2-G418
Plasmid#158459PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hPAX3 (#395)
Plasmid#78335PurposeOverexpression of human PAX3 ORF with a N-terminal FLAG tagDepositorInsertPAX3 (PAX3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-mFoxJ1
Plasmid#122965PurposeExpresses Flag-tagged mouse FoxJ1 in mammalian cellsDepositorInsertmouse FoxJ1 (Foxj1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-c5X-MAP
Plasmid#66999PurposeExpresses malE-MAP in E. coliDepositorInsertmalE-MAP
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA
Plasmid#51095PurposeCre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophoreDepositorInsertChIEF(E162A/T198C)
UseAAVTagsP2A-dTomatoExpressionMammalianMutationE162A/T198CPromoterEF1aAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only