We narrowed to 1,590 results for: PTS

1. pcDNA5 FRT TO myc Separase C2029A

   Plasmid

   #59822

   Purpose

   Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LDB204

   Plasmid

   #185426

   Purpose

   Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

   Depositor

   Ken Belanger, Laura Davis

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 FXFG-repeats domain expressed

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECFP-N2-COL1A1

   Plasmid

   #66603

   Purpose

   Expresses procollagen 1 alpha 1 as a CFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-AtAFB2-mTagBFP2

   Plasmid

   #179889

   Purpose

   Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   AtAFB2 (AFB2 Synthetic, Mustard Weed)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA CrkII Y221F

   Plasmid

   #50732

   Purpose

   mammalian expression of CrkII Y221F mutant

   Depositor

   Kenneth Yamada

   Article

   Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

   Insert

   CrkII Y221F (CRK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y221F

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-SRGAP3FBAR-EGFP

   Plasmid

   #168506

   Purpose

   Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

   Depositor

   Franck Polleux

   Article

   Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

   Insert

   SRGAP3-FBAR (SRGAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-492

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. cmamxA1-GFP

   Plasmid

   #107195

   Purpose

   Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   cmannexin A1
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-bSV-831-1281

   Plasmid

   #40962

   Depositor

   Elizabeth Luna

   Article

   Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

   Insert

   supervillin (SVIL Bovine)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-830 and 1282-1792

   Promoter

   Available since

   Dec. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

9. KBB394

   Plasmid

   #185086

   Purpose

   NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Nma111 KKR 9-11 AAA; KRK 28-30 AAA

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET-10-mCherry-R11b

    Plasmid

    #179891

    Purpose

    Donor with homologous arms for Rab11b to knock in mCherry

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. OfCasp3a

    Plasmid

    #166053

    Purpose

    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Orbicella faveolata caspase-3a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PaCasp7a

    Plasmid

    #166051

    Purpose

    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Porites astreiodes caspase-7a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. INPP5A / type I IP3 5-phosphatase from dog thyroid

    Plasmid

    #131767

    Purpose

    Expression in bacteria or COS-7 cells

    Depositor

    Christophe Erneux

    Article

    Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

    Insert

    INPP5A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC407 - pAAV EF1a eGFP

    Plasmid

    #60058

    Purpose

    An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    enhanced Green Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM2-hPMCA4b

    Plasmid

    #47759

    Purpose

    mammalian expression of human PMCA4b

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA4b (ATP2B4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcdna nesprin TS

    Plasmid

    #68127

    Purpose

    nesprin tension sensor

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    mouse nesprin mini 2G (Syne2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP hsp70

    Plasmid

    #15215

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna nesprin HL

    Plasmid

    #68128

    Purpose

    nesprin headless control

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    nesprin-2 (Syne2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted actin-binding domain

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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