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pRET.IS.IRES-EGFP N2

Plasmid

#1834

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pRET.IIS.IRES-EGFP

Plasmid

#1838

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-GFP-ILVBLpA-G10C

Plasmid

#120206

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA-G10C-C21G

Plasmid

#120207

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-del

Plasmid

#120200

Purpose

human RPS5 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-m1r

Plasmid

#120197

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-m1

Plasmid

#120196

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-del

Plasmid

#120193

Purpose

human CENPB poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA-del

Plasmid

#120204

Purpose

human TFE3 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA-G9C-C19G

Plasmid

#120203

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA-G9C

Plasmid

#120202

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1-CENPB

Plasmid

#120208

Purpose

gRNA targeting the stem loop of CENPB poly(A) site

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 WWE-PARP

Plasmid

#192550

Purpose

E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Use

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Use

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 mouse PARG (439-959)

Plasmid

#132615

Purpose

expresses mouse PARG(439-959) in E. coli.

Depositor

Wenqing Xu

Article

Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

Insert

PARG (439-959)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C204

Plasmid

#174358

Purpose

For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge