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Showing: 1081 - 1120 of 3272 results

  1. mBFP-APPP1-mGFP

    Plasmid
    #196695
    Purpose
    As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity
    Depositor
    Martino Calamai
    Article
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Use
    Tags
    mEGFP and mtagBFP2
    Expression
    Mammalian
    Mutation
    Lys to Val substitution at position 612
    Promoter
    CMV
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196695-plasmid-map-sequence-id-386451

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  3. AP-APP

    Plasmid
    #196706
    Purpose
    Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Article
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Use
    Tags
    AP, Avitag and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196706-plasmid-map-sequence-id-386447

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  4. pCB268

    Plasmid
    #53363
    Purpose
    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.
    Depositor
    Alexander Varshavsky
    Article
    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:
    Inserts
    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    Use
    Tags
    13xMyc, Flag, and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. mZO1-1 shRNA

    Plasmid
    #37215
    Depositor
    Alan Fanning
    Insert
    mouse ZO-1 shRNA (Tjp1 Mouse)
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 12, 2012
    Availability
    Academic Institutions and Nonprofits only
    37215-plasmid-map-sequence-id-176983

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  7. SGEP-shRLuc-308

    Plasmid
    #188667
    Purpose
    control shRNA
    Depositor
    Lewis Cantley
    Insert
    shRLuc
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188667-plasmid-map-sequence-id-375067

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  8. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  9. pAAV CAG Barcode5

    Plasmid
    #229067
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode5
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229067-plasmid-map-sequence-id-455090

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  10. pAAV CAG Barcode4

    Plasmid
    #229065
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode4
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229065-plasmid-map-sequence-id-455089

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  11. pAAV Syn Barcode13

    Plasmid
    #226187
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode13
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226187-plasmid-map-sequence-id-447869

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  12. pRNF219.1.0-gDNA

    Plasmid
    #132464
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RNF219
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132464-plasmid-map-sequence-id-267084

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  13. pOSR1.1.0-gDNA

    Plasmid
    #176381
    Purpose
    Expresses spCas9 and gRNA sequence targeting OSR1
    Depositor
    Kevin White, Barbara Stranger
    Insert
    OSR1 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176381-plasmid-map-sequence-id-347393

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  14. pOTX2.1.0-gDNA

    Plasmid
    #176376
    Purpose
    Expresses spCas9 and gRNA sequence targeting OTX2
    Depositor
    Kevin White, Barbara Stranger
    Insert
    OTX2 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176376-plasmid-map-sequence-id-347362

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  15. PX458_TGIF2_1

    Plasmid
    #72374
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    TGIF2 (TGIF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72374-plasmid-map-sequence-id-134714

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  16. pX458_mCherry-NPM1-gRNA for HCT116

    Plasmid
    #238249
    Purpose
    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus
    Depositor
    Denes Hnisz
    Insert
    NPM1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238249-plasmid-map-sequence-id-474417

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  17. HEY2.1.1-gDNA

    Plasmid
    #175873
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    HEY2-Nickase1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175873-plasmid-map-sequence-id-346224

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  18. SGEP-sh-Hs-PHGDH-67

    Plasmid
    #188671
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188671-plasmid-map-sequence-id-375112

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  19. pCHCHD3-N2.1.2-gDNA

    Plasmid
    #175858
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    CHCHD3-Nickase2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    175858-plasmid-map-sequence-id-346387

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  20. pOCC102-MBP-eIF4G-213

    Plasmid
    #219974
    Purpose
    eIF4G-213 protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-213 (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    213VKLR216 to 213AAAA216
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219974-plasmid-map-sequence-id-436799

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  21. pCfb13209

    Plasmid
    #219884
    Purpose
    The base plasmid of TUNEYALI for TF19
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    219884-plasmid-map-sequence-id-435606

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  22. pCfb13229

    Plasmid
    #219904
    Purpose
    The base plasmid of TUNEYALI for TF39
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF39 (YALI1_B16808g) and homologous arm matching TF39
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219904-plasmid-map-sequence-id-435474

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  23. pCfb13212

    Plasmid
    #219887
    Purpose
    The base plasmid of TUNEYALI for TF22
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219887-plasmid-map-sequence-id-435385

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  24. pPHTF2.1.0-gDNA

    Plasmid
    #132465
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PHTF2 (PHTF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132465-plasmid-map-sequence-id-267085

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  25. PX458_ZNF3_2

    Plasmid
    #72364
    Purpose
    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72364-plasmid-map-sequence-id-134647

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  26. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid
    #188681
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188681-plasmid-map-sequence-id-375018

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  27. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132471-plasmid-map-sequence-id-267178

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  28. pJR255

    Plasmid
    #78549
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CMV
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78549-plasmid-map-sequence-id-191088

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  29. pJK217

    Plasmid
    #72471
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72471-plasmid-map-sequence-id-135051

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  30. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  31. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110426-plasmid-map-sequence-id-213399

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  32. pML107-VHT1

    Plasmid
    #232898
    Purpose
    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VHT1 gRNA (VHT1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232898-plasmid-map-sequence-id-462142

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  33. ipUSEPR-sg-Hs-CDK1

    Plasmid
    #188684
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgCDK1 (CDK1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188684-plasmid-map-sequence-id-375070

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  34. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

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  35. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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  36. pML104-HygMx4-URA3-3

    Plasmid
    #232886
    Purpose
    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232886-plasmid-map-sequence-id-462214

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  37. pML104-HIS3-3

    Plasmid
    #232882
    Purpose
    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232882-plasmid-map-sequence-id-462144

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  38. pML107-ZIM17

    Plasmid
    #232901
    Purpose
    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ZIM17 gRNA (ZIM17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232901-plasmid-map-sequence-id-462145

    Enlarge

  39. pNSU299_RPL22A

    Plasmid
    #166079
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL22A (RPL22A Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166079-plasmid-map-sequence-id-329605

    Enlarge

Showing: 1081 - 1120 of 3272 results