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Showing: 1351 - 1400 of 18908 results

  2. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

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  17. 50_p5E_UBI_loxTagBFP

    Plasmid
    #172518
    Purpose
    p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision
    Depositor
    Jean Giacomotto
    Article
    Guo et al bioRxiv 2024.02.22.581357
    Insert
    ubiquitin promoter, TagBFP, loxP sites (inversion)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    172518-plasmid-map-sequence-id-344833

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  19. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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  20. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  23. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Expression
    Mutation
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  25. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid
    #113685
    Purpose
    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    inverted de-catalyzed SaCas9
    Use
    AAV, CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    NLS and VP64
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113685-plasmid-map-sequence-id-232027

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  30. pCALNL-DsRed

    Plasmid
    #13769
    Purpose
    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    DsRed2
    Use
    Cre/Lox; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13769-plasmid-map-sequence-id-7758

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  33. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

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  39. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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  40. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  41. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid
    #230815
    Purpose
    AAV cloning vector for CRE dependent expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230815-plasmid-map-sequence-id-466637

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  42. pMLS716

    Plasmid
    #188888
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Use
    Tags
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188888-plasmid-map-sequence-id-370784

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  44. pDS66

    Plasmid
    #48364
    Purpose
    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48364-plasmid-map-sequence-id-70059

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  45. pDS67

    Plasmid
    #48365
    Purpose
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48365-plasmid-map-sequence-id-71676

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  46. pDS68

    Plasmid
    #48366
    Purpose
    GFP-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    GFP-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48366-plasmid-map-sequence-id-71698

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  47. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

    Plasmid
    #168787
    Purpose
    Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Type
    Empty backbone
    Use
    Cre/Lox and Synthetic Biology; Expression in fila…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168787-plasmid-map-sequence-id-353783

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  48. pDS69

    Plasmid
    #48367
    Purpose
    FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48367-plasmid-map-sequence-id-71699

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  50. pGECPL.BC10.MG

    Plasmid
    #223822
    Purpose
    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.
    Depositor
    Dawid Nowak
    Article
    Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/
    Insert
    Ef1a-Driven Cre-P2A-FLuc
    Use
    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    223822-plasmid-map-sequence-id-443651

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Showing: 1351 - 1400 of 18908 results