We narrowed to 1,873 results for: FRT

1. pcDNA5/FRT/TO GFP HSPA1A

   Plasmid

   #19483

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA1A (HSPA1A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO GFP DNAJB9

   Plasmid

   #19505

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB9 (DNAJB9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-FLAG-CHK1

   Plasmid

   #86875

   Purpose

   To express CHK1 with a FLAG tag at N-terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   CHEK1 (CHEK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO GFP HSPA8

   Plasmid

   #19487

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA8 (HSPA8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFRT/TO/HIS/FLAG/HA-HNRNPU

   Plasmid

   #38068

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

   Plasmid

   #118375

   Purpose

   Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT/TO-APT1-LaG16-3W

   Plasmid

   #234424

   Purpose

   Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO-GAlpha13-RLuc8

   Plasmid

   #140986

   Purpose

   Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha13-RLuc8 (GNA13 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid

    #196223

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5B-FRT-control-APEX

    Plasmid

    #73187

    Purpose

    Expresses "control"-APEX (cytosolic) in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    control-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    changed glycine 2 to alanine

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyBac CMV-GFP-FRT

    Plasmid

    #92078

    Purpose

    production of transgenic animal and recombination using flipase

    Depositor

    Jae Yong Han

    Article

    Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

    Insert

    CMV-eGFP and TK-Neo between FRT3 and FRT
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO_Lyn11-HaloTag9

    Plasmid

    #175543

    Purpose

    Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD

    Plasmid

    #233363

    Purpose

    CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    Kinprola_PKA-mEGFP-MTBD
    

    Use

    Tags

    mEGFP-MTBD

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5/FRT-ChemoG-CaM

    Plasmid

    #193816

    Purpose

    CMV overexpression of ChemoG-CaM in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-CaM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:E116D, A206K; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

    Plasmid

    #196224

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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