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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

S269D

Promoter

TRE3G

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-HA-c3xFLAG

Plasmid

#215691

Purpose

Produces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tag

Depositor

Melinda Brindley

Article

Willard et al Pathogens. 2018 Dec 26;8(1):1. doi: 10.3

Insert

LASV GP-HA-FLAG

Use

Tags

HA-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-LASV-GPC-c3xFLAG

Plasmid

#215689

Purpose

Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP-FLAG

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

Plasmid

#210612

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdhV5-intronless-5BoxB_X

Plasmid

#147911

Purpose

Insect Expression of DmAdh-intronless-5BoxB

Depositor

Elisa Izaurralde

Insert

DmAdh-intronless-5BoxB (Adh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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OTUB1/pRetroX-IRES-DsRed#10

Plasmid

#180279

Purpose

Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

human Otubain-1 (OTUB1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-no_intron

Plasmid

#127597

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

1xFLAG-MCP expression cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-HSPE-sh1

Plasmid

#92033

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #1 to HPSE (Hpse Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSIREN-RetroQ-HSPE-sh2

Plasmid

#92034

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #2 to HPSE (Hpse Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-SIRPT1-Jatropha curcas

Plasmid

#75327

Purpose

GST fusion Jatropha curcas Sacsin 1-1304

Depositor

Kalle Gehring

Insert

Sacsin Jatropha-curcas 1-1304

Use

Tags

GST-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Jatropha curcas

Plasmid

#75359

Purpose

Nt His-tag Jatropha curcas Sacsin 1-1304

Depositor

Kalle Gehring

Insert

Sacsin Jatropha-curcas 1-1304

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRRP51A+intron-LacZ (pHZ18)

Plasmid

#33131

Depositor

Michael Rosbash

Article

Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14

Insert

RP51A+intron-LacZ (RPS17A Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only