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Showing: 541 - 560 of 1591 results

  1. pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP

    Plasmid
    #220828
    Purpose
    Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1-P2A-NSP5(io)-GS-smURFP
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220828-plasmid-map-sequence-id-444017

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  2. pEXP(FLAG/HA-NSP1-K164-H165A)

    Plasmid
    #188782
    Purpose
    Expresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)
    Depositor
    Markus Hafner
    Article
    Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do
    Insert
    FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
    Use
    Frt site to insert into hek293 t-rex genome
    Tags
    FLAG/HA
    Expression
    Mammalian
    Mutation
    Changed Lysine 164 to Alanine and Histidine 165 t…
    Promoter
    CMV Dox inducible
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    188782-plasmid-map-sequence-id-372761

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  7. PDEST-pSP172BSSPE-pFOGc::RfA-ECFP

    Plasmid
    #186403
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186403-plasmid-map-sequence-id-373700

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  8. pDEST-pSP172BSSPE-Swa1::RfA-ECFP

    Plasmid
    #186402
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186402-plasmid-map-sequence-id-373699

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  9. pDEST-pSP172BSSPE-pFOGc::RfA-venus

    Plasmid
    #186401
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186401-plasmid-map-sequence-id-373698

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  10. pDEST-pSP172BSSPE-pFOGc::venus-RfA

    Plasmid
    #186400
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186400-plasmid-map-sequence-id-373697

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  11. pDEST-pSP172BSSPE-Swa1::venus-RfA

    Plasmid
    #186398
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186398-plasmid-map-sequence-id-373600

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  13. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186413-plasmid-map-sequence-id-369044

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  18. PIESP15-COMP-blac-flag-his

    Plasmid
    #110958
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    parasite-infected erythrocyte surface protein (PIESP15) (PF3D7_0103900 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110958-plasmid-map-sequence-id-223542

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  19. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Article
    Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159390-plasmid-map-sequence-id-310625

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Showing: 541 - 560 of 1591 results