164,609 results

1. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS hTSP-5

   Plasmid

   #13002

   Depositor

   Jack Lawler

   Article

   Newton et al Genomics. 1994 Dec . 24(3):435-9.

   Insert

   COMP (COMP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pSC189

   Plasmid

   #32114

   Depositor

   Eric Rubin

   Article

   Chiang et al Gene. 2002 Aug 21;296(1-2):179-85.

   Type

   Empty backbone

   Use

   Transposon delivery vector

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GADD45 WT-luc

   Plasmid

   #8356

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   GADD45 promoter WT (GADD45A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30-2-GPD1

   Plasmid

   #83911

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN4-EF2-ABCG2-IRES-Neo

   Plasmid

   #25983

   Depositor

   Ren-he Xu

   Article

   Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

   Insert

   ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
   

   Use

   Lentiviral

   Tags

   EF1-apha promoter and IRES-Neo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-RAPH1ΔTBS

   Plasmid

   #194859

   Purpose

   Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 2-92 deleted

   Promoter

   CMV

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-mBAF45a IRES GFP

    Plasmid

    #17880

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45a (Phf10 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-FlaviA-mNeptune-puro

    Plasmid

    #140091

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-mNeptune
    

    Use

    Lentiviral; High expression

    Tags

    mNeptune

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPR141-Tango

    Plasmid

    #66318

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR141 (GPR141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pSD5

    Plasmid

    #197289

    Purpose

    Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans

    Depositor

    Bogumil Karas

    Article

    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

    Insert

    NptII
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Use

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EBFP2-VASP-5

    Plasmid

    #55251

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    VASP (Vasp Mouse)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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