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pcDNA-h-mmPtch1-B-HA-TagBFP

Plasmid

#120910

Purpose

Expresses BFP tagged Ptch1-B

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-mCherry

Plasmid

#65283

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHLP700

Plasmid

#203742

Purpose

C-terminal tagging of target proteins with AID*/3xHA degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain codons 71-114

Use

Pcr amplification template for gene tagging

Tags

3xHA

Promoter

none

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-HBP1

Plasmid

#16178

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

HMG-box transcription factor 1 (HBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pET15b-sfGO-Matryoshka-LE-LP

Plasmid

#100018

Purpose

Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

cpsfGFP-LSSmOrange

Tags

6x HIS tag

Expression

Bacterial

Mutation

Leu and Glu N-term linker and Leu and Pro C-term …

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV miRNA control plasmid

Plasmid

#31508

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pAAV_CaMKIIa_SERT_EYFP

Plasmid

#198737

Purpose

Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

Depositor

Lin Tian

Article

Vargas et al Science. 2023 Feb 17;379(6633):700-706.

Insert

Serotonin transporter and yellow fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART2

Plasmid

#133250

Purpose

Express FLAG-tagged MCART2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART2 (SLC25A52 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pmRFP-N2 (1390)

Plasmid

#62049

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
CDK4-luciferase full length (2135)

Plasmid

#86656

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

2120 base pairs including up to coding nucleotide…

Promoter

CDK4

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPAICS-2XStrep

Plasmid

#125683

Purpose

expresses human PAICS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PAICS (PAICS Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pmcherry α-catenin M319G/R326E

Plasmid

#178648

Purpose

express α-catenin with M319G/R326E mutation

Depositor

Daniel Conway

Article

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

M319G/R326E

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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