We narrowed to 12,326 results for: NSI

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

Type

Empty backbone

Tags

mNeonGreen11

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1

Plasmid

#244200

Purpose

Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (cJun homodimer binding sites)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

cJun-responsive promoter (6 cJun homodimer binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP and His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW91013

Plasmid

#222450

Purpose

Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-VGAT-pHluorin

Plasmid

#207467

Purpose

Lentiviral expression of VGAT-pHluorin

Depositor

Robert Edwards

Article

Jain et al Proc Natl Acad Sci U S A. 2023 Oct 17;12

Insert

VGAT (Slc6a12 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pArcCreER

Plasmid

#46389

Depositor

Liqun Luo

Article

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Arc (Arc Mouse)
CreERT2

Use

Mouse Targeting

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 CA-LRP6-GFP

Plasmid

#29682

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

LDL-receptor related protein 6 (LRP6 Human)

Tags

GFP

Expression

Mammalian

Mutation

extracellular region deleted

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Luciferase:Tnos (GB0110)

Plasmid

#68216

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCHW101008

Plasmid

#222453

Purpose

Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::OsL5::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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