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pMuLE ENTR MCS R4-R3

Plasmid

#62086

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-MKK6-K82M

Plasmid

#21583

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lysine 82 to methionine

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-SdeA

Plasmid

#80837

Purpose

Bacterial expression of SdeA

Depositor

Zhao-Qing Luo

Article

Qiu et al Nature. 2016 May 5;533(7601):120-4. doi:

Insert

SdeA

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
pLS-CG

Plasmid

#12161

Depositor

Inder Verma

Article

Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

Insert

CMV-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Myo19

Plasmid

#127612

Purpose

GFP-tagged Myosin-19

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Myo19

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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px330 Rosa26 Gu1 (LH416)

Plasmid

#99629

Purpose

Plasmid used to target the wildtype Rosa26 allele with Cas9

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Cas9

Use

CRISPR

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR L3-4XeGFP-Myc-L2

Plasmid

#32316

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

4XeGFP-Myc

Use

Gateway multisite entry vector

Tags

Myc

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pSBDEST.IP

Plasmid

#79472

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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TU#712

Plasmid

#15280

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzyfp

Expression

Worm

Mutation

S65G, V68L, S72A, and T203Y

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pCaST-dVP16AD

Plasmid

#15306

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

dVP16AD-ZIP+

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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Anti-GluA2 [L21/32R]

Plasmid

#177480

Purpose

Mammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Gria2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R]

Plasmid

#177438

Purpose

Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PCK2

Plasmid

#20559

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCK2 (PCK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PLK2

Plasmid

#20590

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK2 (PLK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag BMX

Plasmid

#20431

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BMX (BMX Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
ATF4 7: 5’3’ATF4.GFP

Plasmid

#21854

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Vector derived viral termination and poly-adenyla…

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only