We narrowed to 19,813 results for: INO

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hRAD18-EGFP

Plasmid

#68824

Purpose

Expresses human RAD18 tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 tagged with EGFP (RAD18 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMBEC6

Plasmid

#187598

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SLC38A9.1

Plasmid

#71869

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-GRK6

Plasmid

#32693

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK6 (GRK6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RPA2

Plasmid

#22893

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

RPA2 (RPA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-mRFP1

Plasmid

#112914

Purpose

Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

mRFP1

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTETRIS-cargo

Plasmid

#126033

Purpose

Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Type

Empty backbone

Use

Luciferase; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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SERPINE1-bio-His

Plasmid

#52077

Purpose

Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SERPINE1 (SERPINE1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

Plasmid

#177850

Purpose

Bacterial Expression of AMPK

Depositor

Karsten Melcher

Article

Yan et al Science. 2021 Jul 23;373(6553):413-419.

Insert

AMPK (a1b2g1)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCas3-Amp

Plasmid

#207998

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR1 (Retro)

Plasmid

#222687

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-AP1/beta1

Plasmid

#218802

Purpose

Mammalian expression o fhuman AP1 b1

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

AP1 b1 (AP1B1 Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-OEP7-mVenus

Plasmid

#213864

Purpose

Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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