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pHAGE-ERGIC1__RANBP17

Plasmid

#116816

Purpose

Lentiviral expression of ERGIC1__RANBP17 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERGIC1__RANBP17

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp2-SH2

Plasmid

#46460

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp2 (BCAR3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 144-259

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA 27

Plasmid

#132396

Purpose

Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-MCM5__LYN

Plasmid

#116828

Purpose

Lentiviral expression of MCM5__LYN fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MCM5__LYN

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin2-SH2

Plasmid

#46416

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin2 (CHN2 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 49-155

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA1_P2A_Hygro_Barcode

Plasmid

#120451

Purpose

Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-WNK1__PRMT8

Plasmid

#116845

Purpose

Lentiviral expression of WNK1__PRMT8 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WNK1__PRMT8

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTH744-CEN-RLuc/slowmaxCFLuc

Plasmid

#38224

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDC42BPB__RGS6

Plasmid

#116810

Purpose

Lentiviral expression of CDC42BPB__RGS6 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDC42BPB__RGS6

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDC42BPB__SYNJ2BP

Plasmid

#116811

Purpose

Lentiviral expression of CDC42BPB__SYNJ2BP fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDC42BPB__SYNJ2BP

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + PIK3R1 sgSTOP

Plasmid

#100714

Purpose

B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PIK3R1 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF12_P2A_Hygro_Barcode

Plasmid

#120498

Purpose

Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Arg-SH2

Plasmid

#46405

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Arg/Abl2 (ABL2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 151-257

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Dapp1-SH2

Plasmid

#46422

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Dapp1 (DAPP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 25-151

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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