We narrowed to 9,942 results for: Uty

1. pTwist-SARS-CoV-2 Spike-deltaC18 A222V (GTG)

   Plasmid

   #212329

   Purpose

   Encodes SARS-CoV-2 spike A222V for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike A222V (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   A222V

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

   Plasmid

   #223145

   Purpose

   Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

   Plasmid

   #223144

   Purpose

   Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

   Plasmid

   #223154

   Purpose

   Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

   Plasmid

   #223155

   Purpose

   Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5-TEX264-SIM1_mut-cGFP

   Plasmid

   #220212

   Purpose

   Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

   Promoter

   CMV/TetO

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-GFP-Aurora B KD

   Plasmid

   #184043

   Purpose

   Expresses H2B-GFP fused to Aurora B kinase dead

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Changed Lysine 65 to Arginine

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Repo-Man-1-890

   Plasmid

   #184272

   Purpose

   Expresses C-terminally truncated GFP-RepoMan

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated Repo-Man after aminoacid 893

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-RepoMan-S893D

   Plasmid

   #204995

   Purpose

   Expresses shRNA resistant GFP-RepoMan S893D Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 893 to Aspartic acid

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSD139A

    Plasmid

    #218129

    Purpose

    Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    luxAB
    

    Tags

    scP22

    Mutation

    W384A, Y386A

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV2_CAG_oROS-HT(C199S)_WPRE

    Plasmid

    #216415

    Purpose

    Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT(C199S)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHES7-NLuc-2A-tdTomato

    Plasmid

    #204348

    Purpose

    Donor vector to tag endogenous pig HES7 locus

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    NLuc-2A-tdTomato
    

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 mOct4-POU

    Plasmid

    #206393

    Purpose

    Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBT9-FHIP

    Plasmid

    #198527

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP (FHIP1B Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent mutations in codons 353 and 651 as well as…

    Promoter

    ADH1

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAT +R111E

    Plasmid

    #187425

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Expression

    Insect

    Mutation

    changed arginine at 118 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSNA-c029

    Plasmid

    #175471

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    L281R, H288A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Tac-STC(5A)

    Plasmid

    #162491

    Purpose

    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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