We narrowed to 24,628 results for: p

1. pShuttle-EGFP-tubulin

   Plasmid

   #24327

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-mTagBFP2-TEV-Strep-His

   Plasmid

   #210824

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mTagBFP2
   

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. cr3Vn

   Plasmid

   #184861

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mito-mGarnet

   Plasmid

   #104309

   Purpose

   codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mito-mGarnet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. M-ST1-sgRNA

   Plasmid

   #48672

   Purpose

   Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
   

   Use

   CRISPR

   Promoter

   hU

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLux76-LasI

   Plasmid

   #73444

   Purpose

   HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL

   Depositor

   Jim Haseloff

   Article

   Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

   Insert

   LasI
   

   Use

   Synthetic Biology

   Promoter

   pLux76

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBN154

   Plasmid

   #86718

   Purpose

   FLP/Frt dual color reporter for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
   

   Expression

   Worm

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTORKm43GWF

   Plasmid

   #112899

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FCK-QuasAr2-mRuby2 eFRET

   Plasmid

   #59174

   Purpose

   Fluorescent membrane voltage sensor

   Depositor

   Adam Cohen

   Article

   Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

   Insert

   QuasAr2-mRuby2 eFRET
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag mTOR S2035T

    Plasmid

    #26604

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

    Plasmid

    #194880

    Purpose

    Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-D4H
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Zic4-3505:GFP

    Plasmid

    #193001

    Purpose

    Plasmid to generate a transgenic Hydra Zic4 reporter line

    Depositor

    Brigitte Galliot

    Article

    Vogg et al bioRxiv 2021.12.22.47383

    Insert

    Hydra Zic4 promoter
    

    Use

    Hydra transgenesis vector

    Tags

    GFP

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWS5398-CRISPRai-HygR

    Plasmid

    #182837

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG460-FRB-greenNFAST

    Plasmid

    #160361

    Purpose

    Expresses FRB-greenNFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    FRB-greenNFAST
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-DsRed-Express2

    Plasmid

    #103999

    Purpose

    Plasmid encoding DsRed-Express2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ333: pU6-MmeFz2_omegaRNA-entry

    Plasmid

    #205267

    Purpose

    Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWS5390-CRISPRai-URA3

    Plasmid

    #182829

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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