We narrowed to 9,259 results for: pho;

1. pSP64TS human TGFbeta-IIR (DM#169)

   Plasmid

   #15012

   Depositor

   Douglas Melton

   Article

   Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

   Insert

   TGF beta type II receptor (TGFBR2 Human)
   

   Use

   Sp6 expression for use in xenopus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pET28-his-3C_Clu3B6p

   Plasmid

   #100093

   Purpose

   expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

   Insert

   14-3-3 sigma human (SFN Human)
   

   Use

   Tags

   HSPB6 peptide and His6

   Expression

   Bacterial

   Mutation

   75-77 residues changed to AAA to facilitate cryst…

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH-HT_ygiF-FL

   Plasmid

   #127795

   Purpose

   Expresses full-length ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG584

   Plasmid

   #40191

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (RNAi resistant) (mex-5 Nematode)
   

   Use

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   Exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pCS1

   Plasmid

   #55749

   Purpose

   Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH651-RLuc/minFLuc

   Plasmid

   #29697

   Depositor

   Tobias von der Haar

   Article

   Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

   Insert

   Firefly Luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. beta4-nAChR (DPM negative control)

   Plasmid

   #86651

   Purpose

   expression of control shRNA

   Depositor

   Jaime Modiano

   Article

   Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

   Insert

   CHRNB4 (control)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRKVSV FRNK

   Plasmid

   #50534

   Depositor

   Kenneth Yamada

   Article

   Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

   Insert

   protein tyrosine kinase, domain
   

   Use

   Tags

   2X VSV

   Expression

   Mammalian

   Mutation

   truncated (NT2186-3268)

   Promoter

   CMV

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. PLL7.0 tgRFP LOV

   Plasmid

   #114463

   Purpose

   Mammalian expression of tgRFP labelled LOV2

   Depositor

   Andrew Woolley

   Article

   Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

   Insert

   LOV2
   

   Use

   Tags

   tgRFP

   Expression

   Mammalian

   Mutation

   G401A(G528A), N411E(N538E) and V289I (V416I)

   Promoter

   CMV

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEX Tom20 mVenus BoPL

    Plasmid

    #114467

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoPL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx TOM20 mVenus BoLL

    Plasmid

    #114465

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoLL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTriEX Tom20 mVenus BoLD

    Plasmid

    #114468

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoLD

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoLD
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSM15

    Plasmid

    #101640

    Purpose

    Encodes the fluorescent protein EG-4 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    EG-4
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a - NDH

    Plasmid

    #89626

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD(P)-dependent oxidoreductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG587

    Plasmid

    #40196

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S458A; exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

17. miR-144-451 Trap

    Plasmid

    #61488

    Purpose

    to knockdown endogenous miR144 and miR451 levels

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-144-451 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pENTR-CrkII

    Plasmid

    #50024

    Purpose

    pENTR vector for mouse Crk-II

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Crk-II (Crk Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pIL2R FAK Y397F

    Plasmid

    #50525

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    IL-2R

    Expression

    Mammalian

    Mutation

    Y397F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. p201N 1514

    Plasmid

    #47025

    Purpose

    Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

    Depositor

    Wayne Parrott

    Article

    Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

    Inserts

    gma-miR1514 recognition sequence
    nptII selectable marker
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    GmUbi and StUbi-3

    Available since

    Aug. 30, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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