173,736 results

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Purpose

Expresses hM4D (Gi) neuronal inhibitor

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM4D (Gi)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

Plasmid

#239974

Purpose

PiggyBac plasmid for inducible PEmax expression and Puromycin selection

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RTP1S

Plasmid

#149364

Purpose

Expresses RTP1S in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

RTP1S (Rtp1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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N174-MCS (Puro)

Plasmid

#81068

Purpose

Lentiviral vector for constitutive gene expression.

Depositor

Adam Karpf

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-Puro

Plasmid

#170069

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

Cas9 guide RNA scaffold with the F+E scaffold modification

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMV306hsp

Plasmid

#26155

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

hsp60 promoter

Use

Mycobacteria integrating vector

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP II (pMIG II)

Plasmid

#52107

Purpose

a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

Plasmid

#230360

Purpose

AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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VPS13A^mCherry

Plasmid

#118758

Purpose

VPS13A internally tagged with mCherry at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

mCherry inserted at position 1372

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only