We narrowed to 15,732 results for: LEA

1. pcDNA3-HA-CUL1 K720A

   Plasmid

   #19939

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 K720A (CUL1 Human)
   

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   CUL1 with a NEDD8 modification mutant

   Available Since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-N3-TYK2

   Plasmid

   #139354

   Purpose

   Expression of TYK2 protein fused with GFP

   Depositor

   Sandra Pellegrini

   Article

   Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAS9PBKS

   Plasmid

   #68371

   Purpose

   CbAkt promoter driven Cas9-2A-GFP (from px458)

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   Cas9
   

   Tags

   2A-EGFP

   Expression

   Mammalian

   Promoter

   CbAkt

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPEP-TEV cNup98 (1-498)

   Plasmid

   #38037

   Depositor

   Roderick Lim

   Article

   Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

   Insert

   Nucleoporin 98 (NUP98 Human)
   

   Tags

   His-tag, 3Cysteine

   Expression

   Bacterial

   Mutation

   amino acids 1-498

   Promoter

   T7

   Available Since

   Sept. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCherry F8-2

   Plasmid

   #186034

   Purpose

   Express VHH F8-2 as a mCherry fusion in mammalian cells

   Depositor

   Isabelle Landrieu

   Article

   Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

   Insert

   VHH F8-2
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-YOD1

   Plasmid

   #85664

   Purpose

   Expression of human YOD1 with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   YOD1 (YOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-BG505.SOSIP-8his

   Plasmid

   #111847

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCin4-3xFLAG-MKL1

    Plasmid

    #19838

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a SUMO Nup53xl 1-320

    Plasmid

    #85998

    Purpose

    bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-PKM2-R399E

    Plasmid

    #122691

    Purpose

    Express His-tagged PKM2 (R399E) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    R399E

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHIV-EF1a-2A-ZsGreen

    Plasmid

    #231985

    Purpose

    Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hAR1-660

    Plasmid

    #89079

    Purpose

    mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

    Depositor

    Elizabeth Wilson

    Article

    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

    Insert

    hAR-1-660 (AR Human)
    

    Expression

    Mammalian

    Mutation

    aa 1-660 (contains exons A,B,C and part of D)

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-PKM2-H391Y

    Plasmid

    #122692

    Purpose

    Express His-tagged PKM2 (H391Y) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    H391Y

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SynI-FlpOn-CreOff-TdTomato

    Plasmid

    #176275

    Purpose

    Viral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-PLAA

    Plasmid

    #85669

    Purpose

    Expression of PLAA with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    PLAA (PLAA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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