We narrowed to 14,200 results for: cas9

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3

Plasmid

#118200

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

Plasmid

#118203

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

Plasmid

#118202

Purpose

CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

Plasmid

#217798

Purpose

Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

Depositor

Benjamin Kleinstiver

Publication

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR and Lentiviral

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

EF1a

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119065

Purpose

Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Tags

HA

Expression

Bacterial

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hMyoD-IRES-dsRed

Plasmid

#66631

Purpose

Co-expresses human MyoD and dsRed

Depositor

Charles Gersbach

Insert

human MyoD IRES dsRed (MYOD1 Human)

Use

Lentiviral

Promoter

hUbc

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Publication

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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JME4390

Plasmid

#129656

Purpose

LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Publication

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#187324

Purpose

Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUDP025

Plasmid

#103874

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

Depositor

Jean-Marc Daran

Publication

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Publication

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTetO-hOSK-CASH-1

Plasmid

#188977

Purpose

A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

Depositor

Sangdun Choi

Publication

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

Doxycycline inducible reprogramming donor cassette

Expression

Mammalian

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pXSE901B

Plasmid

#91713

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

Depositor

Qi-Jun Chen

Publication

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

Plasmid

#115487

Purpose

CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPN10-gCTG

Plasmid

#114385

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

Depositor

Vincent Dion

Publication

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCTG

Use

CRISPR

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSPneoHHgRNAH

Plasmid

#63557

Purpose

Expresses gRNA in Leishmania

Depositor

Greg Matlashewski

Publication

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

guide sequence

Use

Leishmania expression

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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Adeno FT

Plasmid

#101824

Purpose

Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

Depositor

Andrea Ventura

Publication

Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

Insert

U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article