We narrowed to 19,326 results for: Mut

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pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESpuro2-UBXD1-3x HA

Plasmid

#31836

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

UBXD1 (UBXN6 Human)

Tags

3x HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98N-pBabe-puro

Plasmid

#19236

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Asparagin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR(T/A)

Plasmid

#118465

Purpose

Negative-control mutant for sapphireAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2 (100-467)

Plasmid

#14928

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (100-467) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 100-467

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pFUGW-RapACR_T111C-EYFP

Plasmid

#119195

Purpose

Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR fast mutant T111C

Use

Lentiviral

Tags

EYFP

Mutation

changed threonine 111 to cysteine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2 (1-185)

Plasmid

#14929

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (1-185) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 1-185

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112N-pBabe-puro

Plasmid

#19238

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Asparagin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112H-pBabe-puro

Plasmid

#19237

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Histidin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1-del1174-1649_AH

Plasmid

#148904

Purpose

Mammalian Expression of HsXRN1-del1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1-del1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev(T/A)

Plasmid

#118479

Purpose

Negative-control mutant for RAB-AKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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