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pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR153

Plasmid

#226812

Purpose

Tet-inducible lentiviral vector for 2xHA GPR153 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING 368STOP

Plasmid

#102590

Purpose

Lentivector to express STING delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 368

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STINGHAQ

Plasmid

#102587

Purpose

Lentivector to express STING HAQ and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-354STOP

Plasmid

#102589

Purpose

Lentivector to express STING delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 354

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Irx4

Plasmid

#72692

Purpose

Doxycyline regulated expression of Irx4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Irx 4 (IRX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-FLAG-TAOK2

Plasmid

#197862

Purpose

Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

Depositor

Jonathan Morris

Article

Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

Insert

TAOK2 (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Mutation

ins5

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

Plasmid

#102593

Purpose

Lentivector to express STING V155M S366A and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M S366A

Promoter

SFFV

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS-BRaf-ERTm

Plasmid

#24949

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

BRaf-ER (Esr1, BRAF Human, Mouse)

Use

Retroviral

Tags

Myc

Mutation

V600E in BRAF

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT K842M

Plasmid

#154284

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

K842M mutation (Catalytic Inactive)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBpuro_KLF4_ER

Plasmid

#34609

Depositor

Michael Ruppert

Article

Foster et al Oncogene. 2005 Feb 24;24(9):1491-500.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pENTR1A mKate2-hu_ncOGT 5N5A

Plasmid

#154285

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

N322A, N356A, N390A, N424A, N458A mutations (HCF-…

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

BRCA1 3'UTR (BRCA1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only