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HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET-AspS

Plasmid

#159618

Purpose

Expression plasmid for AcpS phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

AcpS

Tags

6HIS

Expression

Bacterial

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GAL-SUV39H2-L

Plasmid

#89136

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTB-HA

Plasmid

#86869

Purpose

To express FNTB with a HA tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTB (FNTB Human)

Tags

HA tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMEDS

Plasmid

#236568

Purpose

Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

Depositor

Shannon Moonah

Article

Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

Inserts

CD/UPRT (negative selection)
NPT II (positive selection)

Tags

3x Myc and FLAG

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSN444

Plasmid

#108331

Purpose

Expresses Sat2 for Nourseothricin resistance selection of C. elegans

Depositor

Shinsuke Niwa

Article

Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

Insert

Streptothricin acetyl transferase 2

Expression

Worm

Promoter

rps-27 promoter

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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HGXPRT

Plasmid

#64201

Purpose

Expression of Plasmodium falciparum HGXPRT

Depositor

Vern Schramm

Article

Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

Insert

Hypoxanthine guanine xanthine phosphoribosyltransferase

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
VPCT-SUV39H2-L

Plasmid

#89137

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to VP16 activation domain

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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MGAT1-GFP

Plasmid

#163647

Purpose

Expresses GFP tagged MGAT1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT

Plasmid

#154271

Purpose

Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

bacterial codon optimized

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSunS

Plasmid

#171672

Purpose

SunS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG36-ChAT

Plasmid

#99069

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ChAT (choline acetyltransferase) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBW105

Plasmid

#111137

Purpose

For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MLS-ratAANAT-3FLAG (Aanat Rat)

Tags

3x Flag

Expression

Yeast

Promoter

Gal1

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAPi

Plasmid

#127548

Purpose

Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment

Depositor

Luis Vidali

Article

Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

Insert

Adenine Phosphorybosyltransferase transcript segment

Use

RNAi; Empty control

Promoter

Ubiquitin

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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