10,100 results

1. pCAG-T7-TALEN(Sangamo)-Destination

   Plasmid

   #37184

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1_V5_FLAG_Ct

   Plasmid

   #194075

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBFv-UAS3

   Plasmid

   #138399

   Purpose

   10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1_TStrp_HA_Nt

   Plasmid

   #194065

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   HA-Twin_Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1-DEST-His

   Plasmid

   #119763

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   10xHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR1_DVK_AE

   Plasmid

   #188946

   Purpose

   Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p{CFD4-EYFP-3xP3::DsRed}

   Plasmid

   #86863

   Purpose

   Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pminTolR4-R2

   Plasmid

   #67711

   Purpose

   mini Tol2 backbone with att4-attR2 cassette

   Depositor

   Nathan Lawson

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBXNH3CA

   Plasmid

   #47069

   Purpose

   FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), AVI-tag, and…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSTARR-seq_fly

    Plasmid

    #71499

    Purpose

    flySTARR-seq screening vector with DSCP core promoter.

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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