We narrowed to 8,358 results for: BLI

1. 3CEG

   Plasmid

   #60107

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   BIRC6:SDC136-F10:C41228 (BIRC6 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. psd44-G13GA

   Plasmid

   #46827

   Purpose

   expression of mutant G protein alpha 13 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA13 (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G225A

   Promoter

   PGK promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pTXB1-CI

   Plasmid

   #51565

   Purpose

   Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

   Depositor

   Francisco Malagon, Sankar Adhya

   Insert

   cI
   

   Use

   Tags

   Intein-Chiting Binding Domanin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22-6His-SUMO-Nsp10

   Plasmid

   #154960

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pMSCV-Nras61K-Puro

   Plasmid

   #49404

   Purpose

   MSCVpuro based vector, expressing Nras mutant 61K.

   Depositor

   Tobias Neff

   Insert

   N Ras K61 (NRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   61K constitutively activated

   Promoter

   MSCV promotor

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA_DiCre2_59.F2

   Plasmid

   #60209

   Purpose

   Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)

   Depositor

   Jean-Paul Herman

   Insert

   Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
   

   Use

   Tags

   FKBP12(F36V) and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   FKBP12 has the F36V mutation; amino acids 19-59 o…

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BAZ2B

   Plasmid

   #39000

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BAZ2B (BAZ2B Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. L57R

   Plasmid

   #34557

   Depositor

   Kevin Truong

   Insert

   L57 (CASP7 Human, A.Sativa (LOV2))
   

   Use

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic domain of caspase-7 was truncated at am…

   Promoter

   CMV, T7

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. CAPZB2-TwinStrepII

   Plasmid

   #83194

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   CAPZB2 (Capzb Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti.puro.MCV.ER.RAZ.1

    Plasmid

    #114381

    Purpose

    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.

    Depositor

    Reety Arora, Sudhir Krishna

    Insert

    MCV NCCR and tumor T antigen regions
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MCV NCCR (viral own promoter)

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4L59

    Plasmid

    #60087

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pINDUCER20-FLAG-hSRF (#799)

    Plasmid

    #78345

    Purpose

    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. GAPDHS

    Plasmid

    #39010

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GAPDHS (GAPDHS Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-Beta2-PAmcherry1

    Plasmid

    #114190

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and PAmcherry1

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL-c5X-BRT17

    Plasmid

    #67000

    Purpose

    Expresses malE-BRT17 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-BRT17
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. isoform PKC Delta (JCE594)

    Plasmid

    #182025

    Purpose

    yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Delta (Prkcd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184294

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid

    #107736

    Purpose

    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

    Depositor

    Rylan Larsen

    Insert

    synaptophysin-GCaMP6s, mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTetO-Kir2.1-IRES-TLZ

    Plasmid

    #32642

    Depositor

    Joseph Gogos, C. Ron Yu, Richard Axel

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    tetO-Kir2.1-IRES-TLZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid

    #139513

    Purpose

    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    CAG rox stop rox td-sfGFP dre reporter
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge