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LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

Plasmid

#219826

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available since

July 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Publication

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-chlorR

Plasmid

#199992

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-Hph

Plasmid

#188741

Purpose

pUC19 plasmid (MCS intact) with Hygromycin selection marker

Depositor

Michael Bromley

Publication

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

Hygromycin

Use

Filamentous fungal expression

Promoter

gpdA

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE49

Plasmid

#90388

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

PPARg/RXR

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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HT110_FCMV_QuasAr6b_citrine

Plasmid

#178823

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Lentiviral

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTU398

Plasmid

#178980

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

Depositor

Stan Brouns

Publication

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

gRAMP

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE144

Plasmid

#118496

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Expression

Bacterial

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE161

Plasmid

#118509

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE166

Plasmid

#118514

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp1x 2A Hygro

Plasmid

#102806

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

Depositor

Jerry Crabtree

Publication

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos14)GFP

Plasmid

#89252

Purpose

Expresses GFP with +14 theoretical charge in E.Coli

Depositor

David Liu

Publication

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-Ts (tsf E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-cTNT-Cre

Plasmid

#209785

Purpose

Expresses Cre by the specific cTNT promoter

Depositor

Yuxuan Guo

Publication

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

Cre

Use

AAV

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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PrimeRoot.v2C-FLP

Plasmid

#201740

Purpose

For DNA insertion test in protoplast

Depositor

Caixia Gao

Publication

Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

ePPE,FLP

Use

CRISPR

Expression

Plant

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Publication

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Tags

T7 tag and 6xHis

Expression

Bacterial

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Publication

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH2B-miRFP720-N1

Plasmid

#197231

Purpose

Expresses the fusion protein of H2B-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-miRFP720

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_QLuc

Plasmid

#183260

Purpose

Express QLuc in mammalian cells

Depositor

Huiwang Ai

Publication

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

QLuc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 WC visual barcode

Plasmid

#158669

Purpose

tagging clonal populations with whole cell mTurquoise2 fluorescent protein

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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