We narrowed to 63,598 results for: cell

1. pMT2-HA-RAPGEF2(S1244A/S1248A)

   Plasmid

   #110163

   Purpose

   Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

   Insert

   RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1244A/S1248A

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor_ANKRD1_KO_Puro

   Plasmid

   #186667

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Puromycin (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER-21-RUNX1-P2A-ERG

   Plasmid

   #97045

   Purpose

   Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1, ETS-Related Gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_YTHDF2_KO_Hygro

   Plasmid

   #186671

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Hygromycin (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_YTHDF2_KO_Puro

   Plasmid

   #186670

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Puromycin (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER-21-HOXA9

   Plasmid

   #97041

   Purpose

   Expresses HOXA9 in mammalian cells

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   HOXA9 (HOXA9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-MYO10-HMM

   Plasmid

   #87256

   Purpose

   Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-FADD-DD

   Plasmid

   #58263

   Purpose

   Lentiviral vector for expressing a truncated form of FADD (lack of death effector domain) in mammalian cells

   Depositor

   Joan Massague

   Article

   Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

   Insert

   FADD (FADD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   version of FADD (FADD-DD) that contains the FADD …

   Promoter

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. HA-Rab8a-DN (T22N)

   Plasmid

   #101049

   Purpose

   Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   Rab8a (RAB8A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   CMV

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTT3-SP-6xHis-PVR(FL)-FLAG

    Plasmid

    #157622

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PVR (PVR Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER-21-HOXA5

    Plasmid

    #97040

    Purpose

    Expresses HOXA5 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Homeobox A5 (HOXA5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-N1-EXO1b-D173A

    Plasmid

    #111622

    Purpose

    Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

    Insert

    Exonuclease 1b D173A (EXO1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINDUCER-21-LCOR

    Plasmid

    #97038

    Purpose

    Expresses LCOR in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIG-hNFATc2/C - AVITEV

    Plasmid

    #74058

    Purpose

    retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-SM

    Plasmid

    #136578

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-E2A-cMyc (Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

    Plasmid

    #192147

    Purpose

    T cell CRISPR lentiviral vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CNTF-HA

    Plasmid

    #195517

    Purpose

    Expresses HA-tagged CNTF in mammalian cells

    Depositor

    Kevin Park

    Article

    Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

    Insert

    Ciliary neurotrophic factor (CNTF Human)
    

    Use

    AAV

    Tags

    HA-tag and NGF signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    UbC promoter with beta-globin intron

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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