We narrowed to 63,680 results for: cloning
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Plasmid#105795PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mClover3ExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pKK-TEV-ProteinA
Plasmid#105788PurposeExpression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-ProteinAExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2-2ndVal mScarlet-I
Plasmid#104028PurposePlasmid encoding 2ndVal mScarlet-IDepositorInsert2ndVal mScarlet-I
UseLow copyExpressionBacterialMutationmScarlet-I with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6-hGRIN2B-CAG-ps-SpCas9
Plasmid#102851PurposeA single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. pyogenes)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40
Plasmid#101062PurposeAAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2-H396K
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMyCA
Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAceteamidaseAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A(H188A)-polyA
Plasmid#101052Purposein vitro transcription (T7 promoter)DepositorAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIRES H2B EGFP
ExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL002
Plasmid#98921PurposepET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.DepositorInsertsPROPS
GCaMP6f
ExpressionBacterialPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLIX-REST
Plasmid#91896PurposeDoxycycline inducible expression of mouse RESTDepositorAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-AT1R-APEX
Plasmid#96847PurposeFor expression of AT1R-APEX in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAT1R-APEX (AGTR1 Human)
ExpressionMammalianAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDF-mcherry1
Plasmid#87144PurposeConstitutive E. coli mcherry ExpressionDepositorInsertmcherry
ExpressionBacterialPromoterConstitutive proDAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.ELREA_746del
Plasmid#82911PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway CloningMutationDeletion of residues 746-750 (ELREA)PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_KRAS_p.G13D
Plasmid#82808PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_HSPA5_WT
Plasmid#82132PurposeGateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DDIT3_WT
Plasmid#82107PurposeGateway Donor vector containing DDIT3 , part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GRvT
Plasmid#87363PurposeExpresses GRvT, a tandem heterodimer green and red fluorescent proteinDepositorInsertGRvT
TagsHis-TagExpressionBacterialPromoterAraBadAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9
Plasmid#84244PurposeExpresses GA-inducible VPR-Sa dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGAI-tagBFP-Sa dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsGAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWUKI 1+2
Plasmid#79879PurposeEncodes Cas1+2 and a CRISPR arrayDepositorPublicationInsertsCas1
Cas2
CRISPR Array
ExpressionBacterialPromoterT7Available sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only