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We narrowed to 13,842 results for: Cre

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Showing: 5721 - 5740 of 13842 results

  4. pCMV_eA3A-NG-nCas9

    Plasmid
    #163536
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eA3A-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163536-plasmid-map-sequence-id-320176

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  5. nSaCas9 I744 miniABEMax V82G

    Plasmid
    #135379
    Purpose
    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSaCas9 I744 miniABEMax V82G
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    (V82G)
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135379-plasmid-map-sequence-id-267815

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  6. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114082-plasmid-map-sequence-id-220504

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  7. CXCR7-DuET

    Plasmid
    #213224
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR7 (ACKR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213224-plasmid-map-sequence-id-430786

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  8. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid
    #159364
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159364-plasmid-map-sequence-id-310911

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  12. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Publication
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

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  13. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Publication
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Tags
    His tag
    Expression
    Bacterial
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  14. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  15. Cenp-B DBD INCENP GFP

    Plasmid
    #45237
    Purpose
    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Publication
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cenp-B-DBD, INCENP-Δcen, GFP
    Tags
    DNA binding domain of CENP-B and GFP
    Expression
    Mammalian
    Mutation
    CEN box deleted from INCENP (aa1-46)
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45237-plasmid-map-sequence-id-84493

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  16. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1)(RTW1352)

    Plasmid
    #114081
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114081-plasmid-map-sequence-id-231038

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  17. pAaePUb::Cas9-2A-Neo

    Plasmid
    #176680
    Purpose
    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)
    Depositor
    Norbert Perrimon
    Publication
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Insert
    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    Use
    Crispr
    Expression
    Insect
    Mutation
    Drosophila codon optimized
    Promoter
    Ae. aegypti poly-ubiquitin (AAEL003888)
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    176680-plasmid-map-sequence-id-348671

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  19. eGFP-KCC2

    Plasmid
    #104075
    Purpose
    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2
    Depositor
    Igor Medyna
    Publication
    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    CMV
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104075-plasmid-map-sequence-id-199373

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Showing: 5721 - 5740 of 13842 results