We narrowed to 59,355 results for: cloning

1. TALEN-ID12-A

   Plasmid

   #41476

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

   Insert

   TALEN-FokI nuclease backbone
   

   Tags

   3xFLAG and WT FokI nuclease domain

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TALEN-ID12-G

   Plasmid

   #41478

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

   Insert

   TALEN-FokI nuclease backbone
   

   Tags

   3xFLAG and WT FokI nuclease domain

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO SSA1

   Plasmid

   #19462

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   SSA1 (SSA1 Budding Yeast)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-GcAMP5

   Plasmid

   #126569

   Purpose

   Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Use

   Gateway entry clone

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCD1C

   Plasmid

   #102954

   Purpose

   gene cloning of human CD1C

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

   Insert

   CD1C cDNA (CD1C Human)
   

   Use

   Cloning

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

   Plasmid

   #197264

   Purpose

   Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8/GW/TOPO-ELK4

   Plasmid

   #98617

   Purpose

   Gateway entry TOPO TA cloning vector for ELK4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Topo ta cloning vector

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8/GW/TOPO-ELK3

   Plasmid

   #98622

   Purpose

   Gateway entry TOPO TA cloning vector for ELK3

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK3 (Elk3 Mouse)
   

   Use

   Topo ta cloning vector

   Mutation

   E167K

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8/GW/TOPO-ELF1

    Plasmid

    #98627

    Purpose

    Gateway entry TOPO TA cloning vector ELF1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELF1 (Elf1 Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNM1325

    Plasmid

    #197144

    Purpose

    CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    SpCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Promoter

    CAG

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-CS-146

    Plasmid

    #197136

    Purpose

    CloneSelect Indel reporter encoding BC-del1 barcode

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CS-196

    Plasmid

    #197138

    Purpose

    CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-CS-117

    Plasmid

    #197134

    Purpose

    CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-CS-193

    Plasmid

    #197137

    Purpose

    CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    mCherry(M1V/M9A)

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-CS-119

    Plasmid

    #197135

    Purpose

    CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINSPECT_SigP:NLuc

    Plasmid

    #197263

    Purpose

    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pABD933

    Plasmid

    #67917

    Purpose

    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    CTG10
    

    Use

    Gateway cloning vector

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pCR8/GW/TOPO-ETS1

    Plasmid

    #98621

    Purpose

    Gateway entry TOPO TA cloning vector for ETS1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETS1 (Ets1 Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/Myc-His(-)-HSulf-1

    Plasmid

    #13003

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 (SULF1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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