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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEM03

Plasmid

#135417

Purpose

Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Expression

Bacterial and Yeast

Promoter

Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A-GFP Donor for iMTr EED

Plasmid

#131325

Purpose

Donor to generate i-MT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-MT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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A365Y-GFP Donor for iWTr EED

Plasmid

#131324

Purpose

Donor to generate i-WT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-WT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-2

Plasmid

#131322

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pME-TCAP

Plasmid

#109549

Purpose

Multisite gateway vector for 5' tagging with telethonin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

telethonin (tcap Zebrafish)

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab41

Plasmid

#109767

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab41

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab41 (rab41 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rab38b

Plasmid

#109757

Purpose

Multisite gateway vector for 3' tagging with Rab38b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38b (rab38b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rab38c

Plasmid

#109758

Purpose

Multisite gateway vector for 3' tagging with Rab38c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38c (rab38c Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rab41

Plasmid

#109759

Purpose

Muscle specific zebrafish expression of mKate2 fused to p3E-Rab41

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab41 (rab41 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab3b

Plasmid

#109760

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab3b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab3b (rab3b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab19

Plasmid

#109761

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab19

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab19 (rab19 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab27b

Plasmid

#109762

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab27b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab27b (rab27b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab31

Plasmid

#109763

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab31

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab31 (rab31 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab38b

Plasmid

#109765

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab38b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38b (rab38b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab38c

Plasmid

#109766

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab38c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38c (rab38c Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab26-DN

Plasmid

#109745

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab26

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab26 (rab26 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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