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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-His6

Plasmid

#51532

Purpose

Express His6-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

Plasmid

#194880

Purpose

Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Zic4-3505:GFP

Plasmid

#193001

Purpose

Plasmid to generate a transgenic Hydra Zic4 reporter line

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4 promoter

Use

Hydra transgenesis vector

Tags

GFP

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-b

Plasmid

#17591

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-b (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–175, 217–942; See Depositor Comments

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Promoter

U1a

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PP244

Plasmid

#78978

Purpose

Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV) (w57-1)

Plasmid

#17389

Purpose

Gateway entry vector for miRNA expression under CMV promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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CAG_mCh_p85a_mCh

Plasmid

#199630

Purpose

FRET acceptor for IGF1R sensor

Depositor

Ryohei Yasuda

Article

Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

Insert

p85a-SH2 (Pik3r1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad3

Plasmid

#15723

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pAdEasy-EGFP-Tubulin

Plasmid

#24411

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
lvl0-5-4xFF4

Plasmid

#229170

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

4xFF4

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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cr3Ec

Plasmid

#184860

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, gam, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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