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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-MYO10-HMM

Plasmid

#87256

Purpose

Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

Plasmid

#157623

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5 (KIR2DL5A Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Puro

Plasmid

#186667

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Puromycin (ANKRD1 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR

Plasmid

#97038

Purpose

Expresses LCOR in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMT2-HA-RAPGEF2(S1244A/S1248A)

Plasmid

#110163

Purpose

Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Mutation

S1244A/S1248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-PVR(FL)-FLAG

Plasmid

#157622

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PVR (PVR Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OS

Plasmid

#136576

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 WT

Plasmid

#182046

Purpose

expression of Sox2

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Promoter

UbC promoter with beta-globin intron

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2

Plasmid

#193351

Purpose

Retroviral vector expressing human SOX2

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL delta K5S

Plasmid

#34594

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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