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GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCas34

Plasmid

#82386

Purpose

sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Puro

Plasmid

#167895

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-LOV

Plasmid

#22033

Purpose

Mammalian expression of mVenus tagged LOV2 domain

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-LOV

Use

Retroviral

Expression

Mammalian

Mutation

Insert length based on Venus (720bp) + LOV (450bp)

Available Since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG mCherry-LDTS-LIRmut

Plasmid

#189003

Purpose

lipophagy-inducing adaptor protein, AAV, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut, mCherry

Use

AAV

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy48

Plasmid

#55186

Purpose

Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-G97 (for GFP)

Plasmid

#130715

Purpose

Plasmid encoding the bacterial expression of LAMA-G97 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-G97

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto_zhang2.0

Plasmid

#167912

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS1D

Plasmid

#13284

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

Plasmid

#81133

Purpose

mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
VAV2 DH/PH/C1 (VAV2 Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713

Plasmid

#167908

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV-PDAP1

Plasmid

#31586

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PDGFA associated protein 1 (PDAP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pZ001 (AacC2c1 locus)

Plasmid

#70166

Purpose

Expresses AacC2c1 full locus

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 locus

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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