Addgene

We narrowed to 13,635 results for: Egf

Showing: 8611 - 8640 of 13635 results

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ATF4 14: 5’ATF4.uORF1&2AUA.GFP

Plasmid

#21861

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codons of uORFs1&2 were converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pMIG II-BTLA-mGFP

Plasmid

#180798

Purpose

Expression of mouse BTLA fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (Btla Mouse)

Use

Retroviral

Tags

mEGFP

Expression

Mutation

Promoter

MSCV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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phNFL-E396K

Plasmid

#132595

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Use

Tags

Expression

Mammalian

Mutation

1186G>A; Glu396Lys

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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A3H HapII-Cas9n-UGI

Plasmid

#119141

Purpose

Base editor made from APOBEC3H Haplotype II

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSR-siKlf5

Plasmid

#41740

Depositor

Jonathan Katz

Article

Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

Insert

Klf5 (Klf5 Mouse)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
LP380

Plasmid

#90206

Purpose

pEGFP-C1 with cDNA encoding HsDLEC1 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

DLEC1 (DLEC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pKF-D2irTNP2AG-T2APuroR-5kwh

Plasmid

#128255

Purpose

Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

hTetR::P2A::EGFP::T2A::PuroR

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV-D2ir promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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phNFL-P440L

Plasmid

#132596

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Use

Tags

Expression

Mammalian

Mutation

1319C>T; Pro440Leu

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pJK2067

Plasmid

#171845

Purpose

C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

Depositor

Judith Kimble

Article

Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

Insert

his-58, eGFP, mCherry

Use

Tags

N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

Expression

Worm

Mutation

boxB wild type and hairpin mutant

Promoter

mex-5 promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

RPL4A

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

SSA1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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phNFL-N98S

Plasmid

#132592

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Use

Tags

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
A3H HapI-Cas9n-UGI

Plasmid

#119140

Purpose

Base editor made from APOBEC3H Haplotype I

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype I-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2320

Plasmid

#36746

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Left (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2321

Plasmid

#36747

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Right (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Use

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196989

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Non-Target

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2080

Plasmid

#228339

Purpose

Doxycycline-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Doxycycline regulatable synthetic promoter (KpTc3…

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2081

Plasmid

#228340

Purpose

Doxycycline-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Doxycycline regulatable synthetic promoter (KpTc5…

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2086

Plasmid

#228341

Purpose

Doxycycline-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Doxycycline regulatable synthetic promoter (KpTc1…

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2092

Plasmid

#228342

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2093

Plasmid

#228343

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2094

Plasmid

#228344

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge