We narrowed to 32,142 results for: eng

1. lentiCas9-EGFP

   Plasmid

   #63592

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Phil Sharp, Feng Zhang

   Article

   Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and P2A

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hAdipoq-GFP

   Plasmid

   #176215

   Purpose

   Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

   Depositor

   Yu-Hua Tseng

   Article

   Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

   Insert

   GFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro-Bcl2

   Plasmid

   #46971

   Purpose

   lentiviral expression of Bcl2

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221 UBR5

   Plasmid

   #81062

   Purpose

   Gateway entry vector encoding full length UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
   

   Use

   Cloning

   Mutation

   K503R

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB_NEmut

   Plasmid

   #123310

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE control sensor GRAB_NEmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE5max-SF3B1-K700E

   Plasmid

   #200317

   Purpose

   Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Inserts

   K700E-ng1+pg2
   PEmax-2A-MLH1dn
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEf1a-superDn29-P2A-GFP

   Plasmid

   #247152

   Purpose

   Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   superDn29-P2A-GFP
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEf1a-goldDn29-P2A-GFP

    Plasmid

    #247153

    Purpose

    Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

    Depositor

    Patrick Hsu

    Article

    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

    Insert

    goldDn29-P2A-GFP
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1-Nanog

    Plasmid

    #28221

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Nanog (NANOG Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    7SL upstream pollII enhancer

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRRAP sgRNA3

    Plasmid

    #138184

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-Cepheid1b-ST-WPRE

    Plasmid

    #214967

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

    Depositor

    Peng Zou

    Article

    Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

    Insert

    Cepheid1b-ST
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-R2Tg-RTmut

    Plasmid

    #238106

    Purpose

    For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    Reverse transcriptase mutant R2Tg, mammalian codon optimized
    

    Use

    For ivt

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKL123

    Plasmid

    #187889

    Purpose

    AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

    Depositor

    Martin Kampmann

    Article

    Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

    Insert

    NFIA-SOX9
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTT3 Unc5C-FLAG

    Plasmid

    #72196

    Purpose

    Expresses full-length Unc5c with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c (Unc5c Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3 Zscan4 promoter

    Plasmid

    #69249

    Purpose

    Luciferase reporter for Dux4 activation of Zscan4 transcription

    Depositor

    Stephen Tapscott

    Article

    Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

    Insert

    Zscan4 (ZSCAN4 Human)
    

    Use

    Luciferase

    Mutation

    The two single nucleotide mismatches found betwee…

    Promoter

    Zscan4

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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