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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-BE4max

Plasmid

#174700

Purpose

Base Editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-BE4max

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Tags

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEN_empty

Plasmid

#174701

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEN

Use

Lentiviral

Tags

myc tag

Expression

Mutation

Promoter

EFS/U6

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEC

Plasmid

#174702

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEC

Use

Lentiviral

Tags

flag tag

Expression

Mutation

UGI-E11K

Promoter

EFS

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA-hNgAgo

Plasmid

#104384

Purpose

human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

Insert

hNgAgo

Use

Tags

3XFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8

Plasmid

#136376

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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V32M,L84M CoV-2 ORF8

Plasmid

#159934

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Use

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

V32M, L84M

Promoter

T7

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2d10-364delta263-295-TEV-STREP

Plasmid

#171830

Purpose

Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

10-364delta263-295

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Tags

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ291

Plasmid

#129671

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Tags

Expression

Plant

Mutation

BthCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ293 (BhCas12b_v4)

Plasmid

#136383

Purpose

Gateway entry clone for BhCas12b_v4

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BhCas12b_v4

Use

CRISPR

Tags

Expression

Plant

Mutation

K846R/S893R/E837G

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_His-TEV-EGFP

Plasmid

#223723

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

EGFP, not monomeric (has A207)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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2BT-His-TEV-cs-LC3b

Plasmid

#197460

Purpose

Bacterial Expression of human LC3b

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

LC3b (MAP1LC3B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IG-ATG3 (H262A)

Plasmid

#212027

Purpose

Expresses untagged ATG3 (H262A) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171838

Purpose

Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

H85E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171839

Purpose

Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K88E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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