We narrowed to 582 results for: Lambda RED
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Plasmid#147875PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-del698-725-F764E-dsRNAres_U
Plasmid#147719PurposeInsect Expression of DmNot3-del698-725-F764E-dsRNAresDepositorInsertDmNot3-del698-725-F764E-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut_T
Plasmid#147635PurposeInsect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mutDepositorInsertHsTNRC6C_1207-1609-M2WA-CIM1-2mut (TNRC6C Human)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S
Plasmid#147517PurposeInsect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAresDepositorInsertHsTNRC6A-M2WA-CIM1-2mut-siRNAres (TNRC6A Human)
ExpressionInsectMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R
Plasmid#147411PurposeInsect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAresDepositorInsertHsTNRC6A-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)
ExpressionInsectMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N
Plasmid#147024PurposeMammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAresDepositorInsertHsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)
ExpressionMammalianMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
Plasmid#146862PurposeInsect Expression of DmPABPC1_del183-258-siRNAresDepositorInsertDmPABPC1_del183-258-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L
Plasmid#146863PurposeInsect Expression of DmPABPC1_del288-359-siRNAresDepositorInsertDmPABPC1_del288-359-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and six silent muta…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L
Plasmid#146864PurposeInsect Expression of DmPABPC1_del364-550-siRNAresDepositorInsertDmPABPC1_del364-550-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and five silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
ExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…Available SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z
Plasmid#148159PurposeInsect Expression of Dm4E-T_1-511-L36AL69ADepositorInsertDm4E-T_1-511-L36AL69A (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A_T
Plasmid#147636PurposeInsect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389ADepositorInsertHsTNRC6C_1207-1609-M2WA-CIM1-2mut-F1389A (TNRC6C Human)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres_S
Plasmid#147516PurposeInsect Expression of HsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAresDepositorInsertHsTNRC6A-M2WA-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)
ExpressionInsectMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEQ111m
Plasmid#40190DepositorInsertEcoRI endonuclease & EcoRI methylase
ExpressionBacterialMutationchanged Glutamic Acid 111 to GlutaminePromoterLambda PLAvailable SinceNov. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBG01 Recombination plasmid
Plasmid#253997PurposeInducible lambdaRED for genomic recombination.DepositorInsertlambdaRED
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pXC3cIfm
Plasmid#193750PurposeExpresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyExpressionBacterialPromoterPLUXAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only