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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_600/pcDNA3

Plasmid

#51982

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta6

Plasmid

#101294

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMTG02-Amp-FRT

Plasmid

#128271

Purpose

Plasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

TetR-LOV-RRRG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1169R

Plasmid

#116899

Purpose

CenpC:mCherry (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Cenp C (partial)
mCherry
CenpC downstream region

Use

Fungal expression (in magnaporthe oryzae)

Tags

Expression

Mutation

truncated, no stop codon and without start codon

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_M2S_AmpR

Plasmid

#119154

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
cp-DdCBE G1333-N

Plasmid

#174556

Purpose

Chloroplast-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJCSUP35(1-122)

Plasmid

#1091

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 122 of SUP35

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(Ca2+)_117/pcDNA3

Plasmid

#51985

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta7

Plasmid

#101295

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

727-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (WT)

Plasmid

#175419

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL1079

Plasmid

#116897

Purpose

CenpC:eGFP (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Cenp C (partial)
eGFP
CenpC downstream region

Use

Fungal expression (in magnaporthe oryzae)

Tags

Expression

Mutation

truncated, no stop codon and without start codon

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTG01-Amp-FRT

Plasmid

#128270

Purpose

Plasmid that produces the following fusion protein: TetR-LOV-TIP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

TetR-LOV-TIP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(Ca2+)_600/pRSETB

Plasmid

#51976

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pJCSUP35(254-684)

Plasmid

#1092

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 254 - 684 of SUP35

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta5

Plasmid

#101293

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(Ca2+)_300/pRSETB

Plasmid

#51977

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-3GS

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
cp-DdCBE G1333-C

Plasmid

#174557

Purpose

Chloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CT-Nano-lantern(ATP1)/pRSETB

Plasmid

#51990

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Tags

6xHis and chloroplast transit peptid

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only