We narrowed to 1,640 results for: plasmid for e coli

1. pMBA323

   Plasmid

   #214751

   Purpose

   To create delta(glmS,nadE) working strains and maintaining their viability

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-DEST

   Plasmid

   #84652

   Purpose

   E. coli expression vector derived from pET32 with Gateway cassette.

   Depositor

   Neil Olszewski

   Type

   Empty backbone

   Use

   Tags

   His, S tag, and Trx Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

   Plasmid

   #191621

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

   Plasmid

   #191628

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

   Plasmid

   #191627

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXD70LacZ7-DadR/DadS-Pdadh-LacZ

   Plasmid

   #191622

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD70LacZ9.1-HcdR-Phcdh-LacZ

   Plasmid

   #191629

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPR220.08

   Plasmid

   #78553

   Purpose

   Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

   Depositor

   Jeffrey Tabor

   Article

   Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

   Insert

   uirS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23108

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNO183

   Plasmid

   #101068

   Purpose

   ppsR2-bphP1 bicistronic operon plasmid

   Depositor

   Jeffrey Tabor

   Article

   Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

   Inserts

   ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
   bphP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   insert guanine at position 430 to fix frameshift …

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid

    #85818

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc oDam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-MfnG-EcYRS-EGFP*

    Plasmid

    #191119

    Purpose

    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP5GP

    Plasmid

    #112610

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a-LIC

    Plasmid

    #62303

    Purpose

    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOP5GT

    Plasmid

    #112611

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOP5MP

    Plasmid

    #112612

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-MBP and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOP5BT

    Plasmid

    #112609

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sc RFC5-pCDFDuet

    Plasmid

    #239202

    Purpose

    Overexpress Sc RFC5 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC5 (RFC5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOP5TT

    Plasmid

    #112613

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-thioredoxin and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOP5BP

    Plasmid

    #112608

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge