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pLex-FLAG-FASTKD4 mutant delta 226

Plasmid

#73621

Purpose

Expression vector containing FASTKD4 mutant delta 226

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

Y616A, L617A, K618A, K620A

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Brr2

Plasmid

#99995

Purpose

Express yeast Brr2 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Brr2 (BRR2 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Rcl1

Plasmid

#99992

Purpose

Express yeast Rcl1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Rcl1 (RCL1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Mutation

Contains amino acids 1-217 of Rcl1

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Kre33

Plasmid

#99989

Purpose

Express yeast Kre33 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
GFP-Rab19

Plasmid

#31741

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab19 (Rab19 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
CJ38 (miR-1006 seed mutant perfect site)

Plasmid

#17771

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1006 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ39 (miR-1003 seed mutant perfect site)

Plasmid

#17772

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1003 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ45 (miR-1003 5' splice site mut)

Plasmid

#17775

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 5' splice mut

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Mutation

5' splice site mut + compsensatory stem

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ47 (U1 snRNA (U1;21d) 3G mutant)

Plasmid

#17776

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Mutation

3G mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ50 (cg11094 utr mut)

Plasmid

#17778

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg11094 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ58 (cg6551 utr mut)

Plasmid

#17784

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg6551 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pSP64T3 BMP2 pro dnmVg1 (DM#182)

Plasmid

#15074

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

Insert

BMP2-Vg1 dom neg

Use

Sp6 expression for use in xenopus

Mutation

dominant negative version of BMP2-Vg1 chimera. Th…

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pDOXOFF 99xGly

Plasmid

#211346

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDOXOFF 15xGly

Plasmid

#211345

Purpose

DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FMRP_P451S

Plasmid

#198693

Purpose

Expression of human FMRP protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FMR1 (FMR1 Human)

Tags

MBP

Expression

Bacterial

Mutation

changed proline 451 to serine

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pACE WT SOX pACEmam1

Plasmid

#192049

Purpose

Expresses WT SOX (pACEmam1)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX

Use

Unspecified

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GFAP-Lyn-mCherry-pHluorin

Plasmid

#187980

Purpose

Membrane targeted cytoplasmic fluorescent pH sensor

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Lyn-mcherry-pHluorin

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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