We narrowed to 7,184 results for: RAP

1. pLJM1 Flag GFP 4E-BP1 Rheb15

   Plasmid

   #112761

   Purpose

   express GFP-4EBP1-Rheb15

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag eGFP and Rheb15

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

   Plasmid

   #58275

   Purpose

   mammalian expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGL-p21UTRm1&2

   Plasmid

   #20880

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pMX-IY LC3A

   Plasmid

   #89290

   Purpose

   For expression of the mammalian Atg8 protein LC3A without a tag

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Retroviral

   Tags

   IRES-YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

   Plasmid

   #167849

   Purpose

   Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10, nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDEST-CMV GFP-LC3B G120

   Plasmid

   #123116

   Purpose

   Expresses EGFP-LC3B G120 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

   Plasmid

   #194459

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-RNF8-Middle-part

   Plasmid

   #64672

   Purpose

   Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Containing only middle part of RNF8 amino acids 1…

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. ND4-FusXTBE-Left TALE

   Plasmid

   #196496

   Purpose

   Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ND4-FusXTBE-Right TALE

    Plasmid

    #196497

    Purpose

    Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-GOLPH3

    Plasmid

    #21688

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pMX-IY LC3B

    Plasmid

    #89291

    Purpose

    For expression of the mammalian Atg8 protein LC3B without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUDR217

    Plasmid

    #113872

    Purpose

    expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA9-MPH2-3 sgRNA10-MAL11
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1 intron 1 gRNA 27

    Plasmid

    #132396

    Purpose

    Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 Flag GFP 4E-BP1 Omp25

    Plasmid

    #112760

    Purpose

    express GFP-4EBP1-Omp25

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Omp25

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167848

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pURD214

    Plasmid

    #113871

    Purpose

    expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA5 HXT13-15-16 sgRNA2-HXT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUDR220

    Plasmid

    #113873

    Purpose

    expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA8-HXT10 sgRNA7-HXT9-11-12
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge