Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,921 results for: pET

Clear keywords
Showing: 5781 - 5800 of 9921 results

  1. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72391-plasmid-map-sequence-id-133988

    Enlarge

  2. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

    Enlarge

  4. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

    Enlarge

  5. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

    Enlarge

  7. pSUMO-YHRC

    Plasmid
    #54336
    Purpose
    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
    Depositor
    Claes Andréasson
    Publication
    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d
    Type
    Empty backbone
    Tags
    6His-SUMO (yeast gene: SMT3)
    Expression
    Bacterial
    Promoter
    T7-LacO
    Available since
    Aug. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    54336-plasmid-map-sequence-id-84530

    Enlarge

  8. NEDD4L

    Plasmid
    #25169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4l.0574.0947 (NEDD4L Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Hect Domain
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  11. SUV39H1

    Plasmid
    #28113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H1 (SUV39H1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains chromo domain only (amino acid residues …
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  14. 6BHD (SETDB1)

    Plasmid
    #119955
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1 (SETDB1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 190-410
    Promoter
    T7
    Available since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119955-plasmid-map-sequence-id-234347

    Enlarge

  17. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120808-plasmid-map-sequence-id-235136

    Enlarge

Showing: 5781 - 5800 of 9921 results