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mKO2-CDK4

Plasmid

#215065

Purpose

Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

mKO2

Expression

Mammalian

Promoter

LTR

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Tre-Tight ifgMosaic Donor vector (LH121)

Plasmid

#99630

Purpose

To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Mutation

(Please see depositor comments below)

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-R617M,K618M,K632M,K633M

Plasmid

#115778

Purpose

Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

Androgen Receptor (AR Human)

Expression

Mammalian

Mutation

R617M, K618M ,K632M, K633M, insertion of Q81 and …

Promoter

CMV

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-hOct4 DE-SV40-Luc

Plasmid

#26279

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Oct4 Distal Enhancer Element (DE) (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

See Depositor Comments below

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP Mps1 DK

Plasmid

#63703

Purpose

Inducible expression of GFP-Mps1 DK fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 47-156, kinetochore binding domain

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:bmp4-pPH:vsvged

Plasmid

#111157

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

Insert

hBmp4 (BMP4 Human)

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

Plasmid

#114613

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TARDBP)

Plasmid

#109323

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TARDBP Human)

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650A

Plasmid

#171252

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650D

Plasmid

#171253

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

Plasmid

#114611

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

Plasmid

#114617

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C

Plasmid

#114622

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 43 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

Plasmid

#114623

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

Plasmid

#114627

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

Plasmid

#114631

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 49 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA mutant

Plasmid

#244882

Purpose

In vitro transcription of mutant human FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

GRCh38, chr2:70300842G>A

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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