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We narrowed to 6,023 results for: cat.2

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Showing: 5801 - 5820 of 6023 results

  1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide2

    Plasmid
    #118182
    Purpose
    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118182-plasmid-map-sequence-id-240444

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  5. mmu-miR-30a natural design (NAT)

    Plasmid
    #107791
    Purpose
    miR-30a RNAi Expression
    Depositor
    Fabio Penna
    Insert
    whole pre-mmu-miR-30a gene (Mir30a Mouse)
    Use
    RNAi
    Tags
    EmGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    107791-plasmid-map-sequence-id-222779

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  6. pNAS1b split mCherry NEDD4 WW2 neg ctrl

    Plasmid
    #112030
    Purpose
    Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112030-plasmid-map-sequence-id-217140

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  7. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pMSCV-HA-shift-IRES-GFP(KaganE21)

    Plasmid
    #52004
    Purpose
    Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker
    Depositor
    Jonathan Kagan
    Article
    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10
    Insert
    MAVS-Shift
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    A 2 nucleotide insertion was introduced between t…
    Available Since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5801 - 5820 of 6023 results