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p370_DG

Plasmid

#234693

Purpose

Golden gate domesticated pET28b with 3C-mNeongreen-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

3C-mNeongreen-His10

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p359_DG

Plasmid

#234702

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p346_DG

Plasmid

#234676

Purpose

Golden gate domesticated pET28b with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p347_DG

Plasmid

#234677

Purpose

Golden gate domesticated pET28b with His10-Thrombin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA43-GG

Plasmid

#208014

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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InterTag-3xL

Plasmid

#186913

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)3-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT017

Plasmid

#180670

Purpose

Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

LacI

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT016

Plasmid

#180669

Purpose

Encodes Z3EV as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

Z3EV

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT001

Plasmid

#180654

Purpose

Encodes pZ3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pZ3

Use

Synthetic Biology

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcS-pep1-C1C2

Plasmid

#178428

Purpose

pep1 as cell penetrating peptide fused to EV membrane binging domain (C1C2)

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

pep1

Expression

Mammalian

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4121

Plasmid

#131745

Purpose

Control ribozyme eGFP-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV ctl

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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SlutrCas9

Plasmid

#163795

Purpose

Expresses SlutrCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZYIntExc

Plasmid

#139673

Purpose

Expression cassette for phiC31 Excisionase in T-DNA vector

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

phiC31 Excisionase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS926

Plasmid

#123052

Purpose

LexA bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexA-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_12_YlpGPD

Plasmid

#117820

Purpose

Promoter pGPD of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpGPD

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A0301

Plasmid

#91005

Purpose

Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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