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We narrowed to 14,439 results for: eng

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Showing: 5801 - 5820 of 14439 results

  4. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid
    #74603
    Purpose
    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
    Depositor
    Ron Vale
    Publication
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    SFFV
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74603-plasmid-map-sequence-id-141268

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  5. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Publication
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pEABR NA 3C FLAG SA

    Plasmid
    #234987
    Purpose
    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EABR-NA
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234987-plasmid-map-sequence-id-468750

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  8. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

    Plasmid
    #179843
    Purpose
    Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP
    Depositor
    Xiaoping Bao
    Publication
    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCND2 (CCND2 Human)
    Use
    Luciferase and Synthetic Biology
    Tags
    eGFP and luciferase
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179843-plasmid-map-sequence-id-354943

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  9. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid
    #169337
    Purpose
    Expression of the LT-Fucci(SCA) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fucci(SCA) (GMNN, CDT1 Human)
    Tags
    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…
    Expression
    Mammalian
    Mutation
    HaloTag9 = HaloTag7-Q165H-P174R
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    169337-plasmid-map-sequence-id-333245

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  10. pcDNA3-sACE2v5-sfGFP

    Plasmid
    #145176
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145176-plasmid-map-sequence-id-292575

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  11. pcDNA3-sACE2v4-sfGFP

    Plasmid
    #145175
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145175-plasmid-map-sequence-id-292620

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  13. pcDNA3-Q842gp120-8his

    Plasmid
    #100932
    Purpose
    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence and Purification tag after K5…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100932-plasmid-map-sequence-id-197608

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  16. pCOCK CM-C02

    Plasmid
    #46563
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pCOCK CM-C18

    Plasmid
    #46560
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available since
    Sept. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pCEP4-myc-PDGFRalpha-V242K

    Plasmid
    #136436
    Purpose
    Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    V242K (disrupts PDGF binding, but still binds HCM…
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136436-plasmid-map-sequence-id-263246

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  19. pAED2

    Plasmid
    #234599
    Purpose
    Fluorescent reporter of the SOS response
    Depositor
    Tanel Tenson
    Publication
    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):
    Inserts
    mScarlet-I
    gfp-mut2
    Use
    Reporter
    Expression
    Bacterial
    Mutation
    GFPmut2 was derived from avGFP with the following…
    Promoter
    Ptet+dnaK P1 and cda
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234599-plasmid-map-sequence-id-467741

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  20. gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217341
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217341-plasmid-map-sequence-id-426767

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Showing: 5801 - 5820 of 14439 results