We narrowed to 9,085 results for: mel
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Plasmid#176550PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionineDepositorInsertyomRuby2
ExpressionYeastPromoterTDH1(GAP)Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-npas4l
Plasmid#164654Purposefor the synthesis of zebrafish npas4l mRNADepositorInsertnpas4l (npas4l Zebrafish)
UseMrna synthesisMutationPlease see depositor commentsPromoterSP6Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2z-etsrp
Plasmid#164655Purposefor the synthesis of zebrafish etsrp mRNADepositorInsertetsrp (etsrp Zebrafish)
UseMrna synthesisAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
ExpressionBacterialAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-mWasabi
Plasmid#176552PurposeExpression of mWasabi for auxotrophic selection in the absence of leucineDepositorInsertmWasabi
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-eCFP
Plasmid#176558PurposeExpression of eCFP for auxotrophic selection in the absence of methionineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-TagRFP657
Plasmid#176560PurposeExpression of TagRFP657 for auxotrophic selection in the absence of lysineDepositorInsertTagRFP657
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK-eCFP
Plasmid#176559PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available SinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010A
Plasmid#176078PurposepBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
Tags3xHAExpressionBacterialAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010B
Plasmid#176079PurposepBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
Tags3xHAExpressionBacterialAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010C
Plasmid#176080PurposepBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
Tags3xHAExpressionBacterialAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010D
Plasmid#176081PurposepBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
Tags3xHAExpressionBacterialAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTR(EF)-tRNA(Q)
Plasmid#170517PurposePCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80DepositorInsertgRNAcore(EF)-tRNA(Q)
UsePcr template vector for amplifying grnacore(ef)-t…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0193_Cq-Rpl40-Cas9
Plasmid#169346PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0193_Cq-Rpl40-Cas9
UseCRISPRExpressionInsectPromoterCPIJ002413Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG422_UV5
Plasmid#165607PurposeVector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1332K and R1333G mutations in SpCas9PromoterlacUV5 driving Cas9 KG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
908I
Plasmid#160241PurposeVanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attBDepositorInsertsVan Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM59
Plasmid#154158PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.DepositorInsertFirefly luciferase
UseLuciferasePromoterEnhancer for the Drosophila melanogaster Metchnik…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM52
Plasmid#154155PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoterDrosophila melanogaster minimal Hsp70 promoter wi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only